![]() Proteome analysis revealed significant changes only at 48 h, showing 48 differentially abundant proteins identified by mass spectrometry procedures. To unravel proteins associated with growth on n-alkanes, proteome of M145-AH after 6, 24, and 48 h of incubation in the Bushnell-Haas (BH) mineral medium containing n-hexadecane as sole carbon source (H condition) and in BH without any carbon source (0 condition) were compared using 2D-differential gel electrophoresis. The resulting engineered strain, M145-AH, can grow on n-hexadecane as sole carbon source. SoCg, was expressed in the non-alkane-degrading actinomycete Streptomyces coelicolor M145. ![]() The alkB gene, encoding an alkane monooxygenase in the actinomycete Gordonia sp. ![]()
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